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Our Mission: Our study involves body mass development of the organism C. elegans. We are attempting to silence the DPY-10 gene, which codes for normal body mass development using RNA interference (as explained on the homepage). A portion of the DPY-10 gene, roughly 290 basepairs, will be inserted into the L4440 vector. Then, the recombinant vector will be inserted into DH5a cells for its higher transformation efficiency. We will pull the new vectors out of the cells with a miniprep and transform the HT115 cells, where the T7 RNA Polymerase in the HT115 cells will bind to the T7 promoters in the ligated L4440 vector to make double stranded RNA. Then, these cells will be fed to the worms. The resulting worms will be short and fat... in theory.

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