Worm+Care!

Home Editor: Eugene Cho



Information concerning Worm Care:

//Caenorhabditis elegans// is a round worm we experimented on during the RNA interference procedure. We are currently growing multiple plates of C. elegans, feeding it OP50 strain of //E. coli// bacteria. Craig C. Mello and Darryl Conte Jr. used //C. elegans// for their Nobel Prize research.

//C. elegans'// optimal breeding temperature is 20 degree Celcius. It has an average generation time of 4 days.

For more information on //Caenorhabditis elegans//: http://en.wikipedia.org/wiki/C_elegans

Life stages of C. elegans. 

__http://openwetware.org/images/f/f3/C._elegans_life_stages.png__

NGM-lite plate recipe: To make 500ml of NGM-lite solution, mix the following ingredients: 1g NaCl 2g Bactotryptone 1.5g KH2PO4 .25g K2HPO4 10g Agar 2.5 mg of cholesterol 500 ml ddH2O (sterilize, autoclave) Pour the plate when the solution is cool enough.

NGM-lite Amp/IPTG plate recipe: Same procedure as the regular NGM-lite solution. After the solution has cooled off after it has been in the autoclave, add the following ingredients: 500ul of 1000x Ampicilin 2 ml of 0.1 M IPTG Shake it well and pour the plate.

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Process of worm chunking: Source:[]

Picture of a Chunked Plate